Aim:
To test if a sample of food contains protein.
Equipment:
Test tube,
sodium hydroxide,
copper sulfate,
food sample.
Method:
1. Place about 2mL of the food sample into a test tube and add 5 drops of sodium hydroxide.
2. Add 5 drops of copper sulfate.
3. Gently shake test tube from side to side.
Results:
For our experiment, the liquid did not change colour. The copper sulfate, which is blue, is supposed to turn green upon being added to the liquid, and then purple once shaken.
This did not happen.
Our liquids, whether or not protein was present, stayed green/blue even when heated.
Discussion:
Protein is made out of amino acids.
The Biruet Test uses an alkaline mixture, composed of copper sulfate. This alkaline mixture turns purple in the presence of peptide bonds, which hold amino acids together.
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